Coding
Part:BBa_K317008
Designed by: Yuta Sakai Group: iGEM10_Tokyo-NoKoGen (2010-10-19)
Antigen 43
Antigen 43 involves in auto-aggregation of the cell. By expressing Antigen 43, the cell will aggregate when the culture solution is stood. Antigen 43 is derived from E. coli genome. The wildtype of Antigen 43 contains 6 PstI sites but this part does not contain any of them. So, this parts can be used as a normal BioBrick which is able to ligate 2 parts by 3A assembly.
The function of this parts is evaluated by BBa_K317024 and BBa_K317026.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 709
Illegal NgoMIV site found at 1933
Illegal NgoMIV site found at 2443
Illegal NgoMIV site found at 2464
Illegal AgeI site found at 1459
Illegal AgeI site found at 2203
Illegal AgeI site found at 2617
Illegal AgeI site found at 2859 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | Antigen 43 |